Researchers in biomedical physics and biology have significantly improved micro-computed tomography with high brilliance x-ray radiation, combining a newly developed microstructured optical grating with new analytical algorithms. For their development, the team around Julia Herzen from Technical University of Munich (TUM) used the beamline P05 of Helmholtz-Zentrum Hereon at DESY's high-brilliance X-ray light source PETRA III. The new approach that is described in the journal Optica makes it possible to depict and analyze the microstructures of samples in greater detail, and to investigate a particularly broad spectrum of samples.

Micro-computed tomography (micro-CT) is an imaging method which generates detailed three-dimensional images of the internal structure of samples with small dimensions. Researchers in biology, medicine or material sciences can use this method to obtain information on the structure and characteristics of tissue and material samples which are important in diagnoses and other analyses.

Micro-CT is based on x-ray images which are reconstructed to form a three-dimensional image. Depending on the sample, different x-ray imaging methods are used in order to achieve the most accurate depiction possible. Here the key parameters are resolution, contrast and the sensitivity of the method used.

X-ray imaging with phase contrast is particularly well-suited for investigating soft tissue. The method employs the refraction of the x-rays caused by the sample's structures in order to obtain contrast for these structures and thus to depict soft tissue in greater detail than it is possible with conventional x-ray methods.

In many phase-contrast methods, optical components modulate the x-rays on their way to the detector, resulting in what is referred to as a diffraction pattern at the detector. "When comparing this pattern with and without the sample in the x-ray beam, the refraction of the x-rays on the sample provides information about its characteristics," says Julia Herzen, professor of Biomedical Imaging Physics at TUM.

Until now inefficient structures such as sandpaper and absorption masks have been used for this type of modulation, but in the meantime a variety of optical gratings are available. "The function of the new optical gratings resembles that of small lenses. The gratings focus the x-rays to form tiny points. This renders the differences in intensity with and without the sample much clearer and makes it possible to visualize even minute differences in the tissue in greater detail," says Herzen.

Physicist Julia Herzen and her team have now introduced a new method for micro-CT with phase contrast using high-brilliance x-ray radiation. The technology is based on a newly developed optical grating referred to as a Talbot Array Illuminator. This new optical element is comparatively easy to produce, is resilient to x-ray radiation and can be used with different energies. This establishes the technically necessary prerequisites for high contrast. The new method enables more efficient use of the radiation dose than with ordinary modulators such as sandpaper and significantly reduces scan times.

"By combining our newly developed Talbot Array Illuminator with new analysis software optimized for the purpose, we've been able to significantly improve imaging and analysis with micro-CT. The new technology is more sensitive than comparable methods in this field. At very high resolutions, it allows to depict soft tissue with higher contrast than previously. High sensitivity is particularly important for example in order to detect fine differences within soft tissue," says Herzen.

The new technology can be used to investigate a particularly broad spectrum of samples. Researchers can even simultaneously depict materials of greatly differing compositions, for example water and oil embedded in stone, which was not possible in the past using conventional methods. This provides crucial advantages over conventional methods not only in medicine and biology, but also opens up new application possibilities in material sciences, for example in geology.

"In contrast to previous approaches, our new method also makes quantitative analysis possible. We can make and compare absolute measurements of the electron density of samples, without the need for any assumptions about the samples," explains Herzen. Further studies will investigate the potential of this new option in a variety of applications.

Scientists from TUM, Hereon and the Universities of Sheffield in Great Britain and Trieste in Italy contributed to this work.


Reference:
High-resolution and sensitivity bi-directional x-ray phase contrast imaging using 2D Talbot array illuminators; Alex Gustschin, Mirko Riedel, Kirsten Taphorn, Christian Petrich, Wolfgang Gottwald, Wolfgang Noichl, Madleen Busse, Sheila E. Francis, Felix Beckmann, Jörg U. Hammel, Julian Moosmann, Pierre Thibault, and Julia Herzen; „Optica“, 2021; DOI: 10.1364/OPTICA.441004

 

Source: Press release from TU Munich